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2X PCR Master mix Solution (i-StarTaq)

Cat.No Capacity Inquire
25166 0.5 ㎖ x 2 vials Inquire

2x PCR Master mix type product with high specificity i-StarTaq™ DNA Polymerase with hot-start function to reduce nonspecific amplification

  • • Ready to Use
       - All reagents required for the reaction are in solution in the tube   
       -  Immediate PCR by adding template and primer
  • • PCR reaction volume control
  • • High specificity and accuracy with hot-start function
  • • High-purity Hot-start Taq DNA Polymerase
  • • Highly reproducible results
  • • Applicable to general PCR, RT-PCR, multiplex PCR and DNA discrimination by PCR (VNTR, STR, RAPD)
  • • Applicable to DNA from cloned DNA to human genomic DNA 

2x PCR Master Mix Solution (i-StarTaqTM) is a product designed to achieve the best results with simple, 2x master mix type in one tube, including i-i-StarTaq™ DNA Polymerase. This product is able to perform PCR by adding template DNA, primer set and D.W only, and gel loading buffer is included, so gel loading can be done without any other treatment. The i-StarTaq™ DNA Polymerase used in this product is a non-antibody-based, chemical-based hot-start enzyme that reduces nonspecific amplification and improves specificity and accuracy. It is a non-specific amplicon such as a nonspecific band or primer dimer It can be used as a solution when the singular band is weakly amplified. The 2x PCR Master Mix Solution (i-StarTaqTM), developed using this i-StarTaq™ DNA Polymerase, is capable of producing the results of high specificity and accuracy as well as the convenience mentioned above and it is highly reproducible.

  • 01Pathogen detection
  • 02Genomic DNA PCR
  • 03Hot-start PCR
  • 04Real-time PCR
  • 05High GC rich, repeat region PCR
  • 06T/A vector cloning
  • 07LOH or MSI analysis related PCR, etc.
Kit Contents
Content 25166
2x PCR Master mix Solution(i-StarTaqTM) 0.5 ml x 2 tubes



Technical Data

Sensitivity Comparison data

In order to compare the sensitivity of 2x PCR Master Mix Solution (i-StarTaqTM) with other products of the same function, 1 Kb DNA fragment PCR was performed by diluting human genomic DNA by concentration. Our kit shows better results.

A, 2x PCR Master mix Solution(i-StarTaq™); B, Competitor Lane M, 1 Kb DNA Marker; lane NC, Negative control; lane 1, 50 ng DNA; lane 2, 25 ng DNA, lane 3, 12.5 ng DNA, lane 4, 6.25 ng DNA, lane 5, 3.125 ng DNA; lane 6, 1.56 ng DNA; lane 7, 0.753 ng DNA

(780 bp), tsh (420 bp), and Irp2 (280 bp) to dilute the E. coli genomic DNA template in order to compare the sensitivity with 2x PCR Master Mix Solution (i-StarTaqTM) As a result of multiplex PCR, we can confirm the superior results compared with other products.

A, 2x PCR Master mix Solution(i-StarTaq™); B, Competitor Lane M, 100 bp DNA Marker; lane NC, Negative control; lane 1, 100 ng gDNA, Lane 2, 50 ng gDNA; lane 3, 25 ng gDNA; lane 4, 12.5 ng gDNA; Lane 5, 6.25 ng gDNA; lane 6, 3.125 ng gDNA; lane 7, 1.5625 ng gDNA

TroubleShooting Guide
QI am conducting a PCR experiment with the virus gene as a template. Non-specific band seems to be floating. Is it possible to remove non-specific band?
AWe recommend our i-StarTaq™. In general, the most common problem encountered in PCR is the amplification of nonspecific bands. Many people use the hot-start method to reduce nonspecific reactions. i-StarTaq™ is a product with hot-start effect, which reduces nonspecific amplification such as nonspecific band or primer dimer. 
QI used i-StarTaq™ to reduce nonspecific reactions, but multi-band appears.
ASome customers use a template more than the recommended amount to increase the amount of PCR product. If an excess of template is added, the template will act as a PCR inhibitor and the PCR reaction may not occur normally. Before the experiment, check the template and check the primer. If there are other parts with the same sequence, multi band may appear.
QIs there a problem with T / A cloning in case of i-StarTaq™? T-cloning is not possible if pfu function is included. Is T-cloning possible?
AOur i-StarTaq ™ DNA Polymerase is a hot start Taq ™ DNA Polymerase and does not use pfu™ DNA Polymerase. As a result, there is no problem with T / A cloning.
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