Maxime™ PCR PreMix (i-Taq)
Dried and aliquoted PreMix Kit of high purity i-Taq ™ DNA Polymerase that displays stable and efficient DNA amplification regardless of template type and reaction conditions
Maxime ™ PCR PreMix Kit (i-Taq™) contains i-Taq™ DNA Polymerase and all components necessary for PCR reaction (dNTP mixture, reaction buffer, etc.) are included in each PCR tube by ALHP system. Because it is in the state, it can remarkably reduce the experimental error to pipetting between the experimenter, and it has excellent reproducibility. It is a product with excellent stability of activity due to the oxidation and moisture prevention of main components in the product by vacuum compression manufacturing method . In addition, this product can perform PCR by adding template DNA, primer set and D.W only, and it can contain gel loading buffer. The i-TaqTM DNA Polymerase used in this product is a thermostable DNA polymerase of 94 kDa, which is expressed in E.coli by cloning the polymerase gene of Thermus aquaticus (strain YT1) Is purified to a high purity and stable and efficient DNA amplification is possible. It is possible to amplify up to 5 Kb from genomic DNA and cDNA. In addition, we optimized the buffer composition so that the best polymerase activity can be obtained regardless of the template type or reaction conditions. Maxime ™ PCR PreMix Kit (i-Taq™) was designed to enable stable and efficient DNA amplification using i-Taq ™ DNA Polymerase as above, It is a product that can be tested very quickly and easily for various samples.
|Maxime™ PCR PreMix Kit(i-StarTaq, for 20 μl rxn)||96 tubes||480 tubes|
Sensitivity comparison data
To compare the sensitivity of MaximeTM PCR PreMix Kit (i-TaqTM), we conducted comparative experiments with products of the same function of other companies. As a result of PCR with 1 Kb primer by dilution of λDNA by concentration by 1/5 concentration, the results are equal to or better than those of other products, as shown in the results.
Lane M, 1 Kb DNA Marker; lane NC, Negative control; lane 1, 200 ng λDNA, lane 2, 40 ng λDNA; lane 3, 8 ng λDNA; lane 4, 1.6 ng λDNA; lane 5, 320 pg λDNA; lane 6, 60 pg λDNA; lane 7, 12 pg λDNA