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PCR

Maxime™ PCR PreMix (i-Taq)

Cat.No Capacity Inquire
25025 96 tubes (20 ㎕) Inquire
25026 96 tubes x 5 ea Inquire
PRODUCT INFORMATION
Description

Dried and aliquoted PreMix Kit of high purity i-Taq ™ DNA Polymerase that displays stable and efficient DNA amplification regardless of template type and reaction conditions

  • • Dried & aliquoted PreMix, All-in-one products
       - All the reagents required for the reaction are in dried pellet in one tube    
       - Immediate PCR by adding template and primer
       - Gel loading electrophoresis is possible because it contains loading dye
  • • Excellent product stability and reproducibility
       - Excellent reproducibility with dried & aliquoted PreMix by ALHP system
       - Excellent stability due to oxidation and moisture prevention by vacuum compression manufacturing method
  • • 94 KDa thermostable DNA polymerase  
  • • High purity Taq DNA Polymerase   
       - Removal of E. coli -derived proteins and DNA that may act as PCR sources
  • • Buffer optimization to show the best polymerase activity regardless of template type or reaction conditions
  • • high reproducible results
  • • Applicable to DNA from cloned DNA to human genomic DNA

Maxime ™ PCR PreMix Kit (i-Taq™) contains i-Taq™ DNA Polymerase and all components necessary for PCR reaction (dNTP mixture, reaction buffer, etc.) are included in each PCR tube by ALHP system. Because it is in the state, it can remarkably reduce the experimental error to pipetting between the experimenter, and it has excellent reproducibility. It is a product with excellent stability of activity due to the oxidation and moisture prevention of main components in the product by vacuum compression manufacturing method . In addition, this product can perform PCR by adding template DNA, primer set and D.W only, and it can contain gel loading buffer. The i-TaqTM DNA Polymerase used in this product is a thermostable DNA polymerase of 94 kDa, which is expressed in E.coli by cloning the polymerase gene of Thermus aquaticus (strain YT1) Is purified to a high purity and stable and efficient DNA amplification is possible. It is possible to amplify up to 5 Kb from genomic DNA and cDNA. In addition, we optimized the buffer composition so that the best polymerase activity can be obtained regardless of the template type or reaction conditions. Maxime ™ PCR PreMix Kit (i-Taq™) was designed to enable stable and efficient DNA amplification using i-Taq ™ DNA Polymerase as above, It is a product that can be tested very quickly and easily for various samples.

Applications
  • 01Amplify Genomic DNA, cDNA to 5 Kb
    예) DD-PCR, DD-RT-PCR, HLA typing, PCR-based DNA typing
  • 02T/A Cloning or Blunt-end Cloning
Kit Contents
Content 25165 25167
Maxime™ PCR PreMix Kit(i-StarTaq, for 20 μl rxn) 96 tubes 480 tubes

 

 

Technical Data

Sensitivity comparison data

To compare the sensitivity of MaximeTM PCR PreMix Kit (i-TaqTM), we conducted comparative experiments with products of the same function of other companies. As a result of PCR with 1 Kb primer by dilution of λDNA by concentration by 1/5 concentration, the results are equal to or better than those of other products, as shown in the results.

Lane M, 1 Kb DNA Marker; lane NC, Negative control; lane 1, 200 ng λDNA, lane 2, 40 ng λDNA; lane 3, 8 ng λDNA; lane 4, 1.6 ng λDNA; lane 5, 320 pg λDNA; lane 6, 60 pg λDNA; lane 7, 12 pg λDNA

TroubleShooting Guide
QCan I adjust the reaction volume to get more amplicon?
AYes. It is recommended to use 20 ~ 50ul total volume as a solution mixed for optimal amplification.
QNon specific amplification phenomenon occurs.
AIt is recommended to raise the annealing temperature. Or the design of the primer is not correct and the amplification may not have occurred well.
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