Maxime™ PCR PreMix (i-MAXⅡ)
Proofreading capability enables high sensitivity, accuracy and low amplification from small size DNA to DNA of more than 20Kb
All components (dNTP mixture, reaction buffer, etc.) necessary for PCR reaction, including i-MAXTM II DNA Polymerase, are included in each PCR tube by the ALHP system, and the dried pellet Because it is in the state, it can remarkably reduce the experimental error to pipetting between the experimenter, and it has excellent reproducibility. It is a product with excellent stability of activity due to the oxidation and moisture prevention of main components in the product by vacuum compression manufacturing method . In addition, this product can perform PCR by adding template DNA, primer set and D.W only, and it can contain gel loading buffer. The i-MAXTM II DNA Polymerase used in this product has the advantages of Taq DNA Polymerase and Pfu DNA Polymerase to overcome the disadvantages of each enzyme and to obtain new functions. In the case of Taq DNA Polymerase, if the template is over 5 Kb, the efficiency of amplification is significantly lowered. Depending on the type of template, DNA template of 10 Kb or more is difficult to amplify. This is because Taq DNA Polymerase has no proof-reading activity, and when mismatched nucleotides are inserted during DNA synthesis, the template fails to polymerize. These defects in Taq DNA Polymerase can be overcome by using a thermostable DNA polymerase with corrective function.
This corrective function is to remove the mismatched (non-complementary) nucleotide when the DNA Polymerase undergoes a polymerization reaction, and correct it with a complementary nucleotide. This activity allows PCR products of more accurate base sequences to be obtained than Taq DNA Polymerase, but allows amplification of longer DNA templates compared to Taq DNA Polymerase. Pfu DNA Polymerase is a typical enzyme of thermostable DNA Polymerase with this corrective function. It is suitable for long size PCR (up to ~ 30 Kb), which is difficult to amplify with general Taq. It has higher fidelity and proof reading function than general Taq DNA Polymerase . However, Pfu DNA Polymerase has the disadvantage that it takes more time to polymerize the DNA due to its poor polymerization efficiency. Maxime ™ PCR PreMix Kit (i-MAX ™ II) can be used for small size (4 ~ 5Kb or less) using i-MAX ™ II DNA Polymerase which takes advantages of Taq DNA Polymerase and Pfu DNA Polymerase. It not only increases the amplification rate but also gives excellent results in the sensitive part. All components required for the PCR reaction are in a dried pellet state in one tube, so you can test very quickly and easily on various samples.
|Maxime™ PCR PreMix Kit(i-MAX II, for 20 μl rxn)||96 tubes|
Various size for amplification efficiency
To observe the amplification efficiency of the Maxime™ PCR PreMix Kit (i-MAX™ II), we have amplified a variety of sizes from 87 bp to 20 Kb. Good amplification efficiency can be seen in all sizes.
[ Panel A ] Using various template
Lane M1, 100bp Ladder DNA Marker; lane M2, 1Kb Ladder DNA Marker; lane 1, 87bp; lane 2,200bp; lane 3, 570bp, lane 4, 1Kb; lane 5, 1.3Kb;
lane 6, 1.8kb; lane 7, 2Kb; lane 8, 2.7Kb; lane 9, 4.5Kb; lane 10, 9Kb; lane 11, 17.5Kb; lane 12, 20Kb
[ Panel B ] Using only human gDNA template
Lane M1, 1Kb Ladder DNA Marker; lane M2, λ/Hind III Marker; lane 1, 1.8Kp; lane 2, 2Kb; lane 3, 2.7Kb, lane 4, 17.5Kb