Description

I-StarMAX™ II DNA with hot-start function and proofreading function for high sensitivity and accurate amplification

• • Dried & aliquoted PreMix, All-in-one products
     - All the reagents required for the reaction are in dried pellet in one tube
     - Immediate PCR by adding template and primer
     - Gel loading electrophoresis is possible because it contains loading dye
• • Excellent product stability and reproducibility   
     - Excellent reproducibility with dried & aliquoted PreMix by ALHP system
     - Excellent stability due to oxidation and moisture prevention by vacuum compression manufacturing method   
• • High accuracy, specificity and low error rate
     - Added Hot-start function of i-StarTaq™ DNA polymerase
     - Proof-reading activity enables amplification of long DNA template
     - High amplification efficiency and sensitivity to amplification of long and short template DNA
• • Cloned DNA, human genomic DNA, as well as GC-rich or looped sequences that are difficult to amplify can be applied to various DNA

Maxime ™ PCR PreMix Kit (i-StarMAX™ II) contains all the components (dNTP mixture, reaction buffer, etc.) necessary for PCR reaction including i-StarMAX™ II DNA Polymerase in each PCR tube by ALHP system Since it is dried pellet state, it can remarkably reduce the experimental error in pipetting between the experimenters. It is very excellent in reproducibility. It is a product with excellent stability of activity due to oxidation and moisture prevention of major components in the product by vacuum compression manufacturing method can. In addition, this product can perform PCR by adding template DNA, primer set and D.W only, and it can contain gel loading buffer.

I-StarMAX™ II DNA Polymerase used in this product is a mixture of hot-start i-StarTaq™ DNA Polymerase and Proofreading DNA Polymerase. It reduces nonspecific reaction and error rate, It is very useful for PCR. It is also useful for demanding template / primer systems and high yield PCR products can be obtained by setting the extension time of PCR to 30 ~ 60 seconds per Kb. i-StarMAX™ ll DNA Polymerase is suitable for high-performance amplification and relatively fast PCR for over 15 Kb of PCR target with proofreading DNA Polymerase alone. Maxime ™ PCR PreMix Kit (i-StarMAX™ II) uses i-StarMAX™ II DNA Polymerase to reduce the nonspecific reaction rate. It has a low error rate due to its proofreading function, In addition, all components required for PCR reaction are in a dried pellet state in one tube, so that it can be tested very quickly and easily for various samples.

Applications

• 01General PCR
• 02Disease diagnostics
• 03SNP analysis
• 04Human genomic DNA for PCR
• 05Multi-plex PCR
• 06TA Cloning & Blunt Cloning, etc.

Kit Contents

Technical Data

To observe the amplification efficiency of the Hot-Star and Long PCR functions of the Maxime™ PCR PreMix Kit (i-StarMAX ™ II), the hot-start function can be confirmed. As a result of the PCR of condition and 20 Kb long PCR, the hot-start function is superior compared with the products of the same function of other companies, so 20 Kb long PCR also shows good amplification efficiency.

[ Panel A Comparison of PCR amplification with supplier A
Lane M1, 100 bp Ladder DNA Marker; lane 1, 161 bp; lane 2, 165 bp; lane 3, 166 bp, lane 4, 181 bp; lane 5, 218 bp; lane 6, 266 bp
[ Panel B ] Amplification of 20Kb PCR fragment
Lane M, λ/Hind III Marker; lane 1, 100 pg lamda DNA; lane 2, 10 pg lamda DNA; lane 3, 1 pg lamda DNA, lane 4, 100 fg lamda DNA; lane 5, 10 fg lamda DNA