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PCR

Maxime™ PCR PreMix (i-StarMAXⅡ)

Cat.No Capacity Inquire
25281 96 tubes Inquire
PRODUCT INFORMATION
Description

I-StarMAX™ II DNA with hot-start function and proofreading function for high sensitivity and accurate amplification

  • • Dried & aliquoted PreMix, All-in-one products
       - All the reagents required for the reaction are in dried pellet in one tube
       - Immediate PCR by adding template and primer
       - Gel loading electrophoresis is possible because it contains loading dye
  • • Excellent product stability and reproducibility   
       - Excellent reproducibility with dried & aliquoted PreMix by ALHP system
       - Excellent stability due to oxidation and moisture prevention by vacuum compression manufacturing method   
  • • High accuracy, specificity and low error rate
       - Added Hot-start function of i-StarTaq™ DNA polymerase
       - Proof-reading activity enables amplification of long DNA template
       - High amplification efficiency and sensitivity to amplification of long and short template DNA
  • • Cloned DNA, human genomic DNA, as well as GC-rich or looped sequences that are difficult to amplify can be applied to various DNA

Maxime ™ PCR PreMix Kit (i-StarMAX™ II) contains all the components (dNTP mixture, reaction buffer, etc.) necessary for PCR reaction including i-StarMAX™ II DNA Polymerase in each PCR tube by ALHP system Since it is dried pellet state, it can remarkably reduce the experimental error in pipetting between the experimenters. It is very excellent in reproducibility. It is a product with excellent stability of activity due to oxidation and moisture prevention of major components in the product by vacuum compression manufacturing method can. In addition, this product can perform PCR by adding template DNA, primer set and D.W only, and it can contain gel loading buffer.

I-StarMAX™ II DNA Polymerase used in this product is a mixture of hot-start i-StarTaq™ DNA Polymerase and Proofreading DNA Polymerase. It reduces nonspecific reaction and error rate, It is very useful for PCR. It is also useful for demanding template / primer systems and high yield PCR products can be obtained by setting the extension time of PCR to 30 ~ 60 seconds per Kb. i-StarMAX™ ll DNA Polymerase is suitable for high-performance amplification and relatively fast PCR for over 15 Kb of PCR target with proofreading DNA Polymerase alone. Maxime ™ PCR PreMix Kit (i-StarMAX™ II) uses i-StarMAX™ II DNA Polymerase to reduce the nonspecific reaction rate. It has a low error rate due to its proofreading function, In addition, all components required for PCR reaction are in a dried pellet state in one tube, so that it can be tested very quickly and easily for various samples.

Applications
  • 01General PCR
  • 02Disease diagnostics
  • 03SNP analysis
  • 04Human genomic DNA for PCR
  • 05Multi-plex PCR
  • 06TA Cloning & Blunt Cloning, etc.
Kit Contents
Content 25281
MaximeTM  PCR PreMix Kit(i-StarMAX II, for 20 μl rxn) 96 tubes

 

 

Technical Data

Comparison of amplification efficiency according to various hot-start PCR conditions and long PCR (20 Kb) conditions (comparison to other companies)

To observe the amplification efficiency of the Hot-Star and Long PCR functions of the Maxime™ PCR PreMix Kit (i-StarMAX ™ II), the hot-start function can be confirmed. As a result of the PCR of condition and 20 Kb long PCR, the hot-start function is superior compared with the products of the same function of other companies, so 20 Kb long PCR also shows good amplification efficiency.

[ Panel A Comparison of PCR amplification with supplier A
Lane M1, 100 bp Ladder DNA Marker; lane 1, 161 bp; lane 2, 165 bp; lane 3, 166 bp, lane 4, 181 bp; lane 5, 218 bp; lane 6, 266 bp
[ Panel B ] Amplification of 20Kb PCR fragment
Lane M, λ/Hind III Marker; lane 1, 100 pg lamda DNA; lane 2, 10 pg lamda DNA; lane 3, 1 pg lamda DNA, lane 4, 100 fg lamda DNA; lane 5, 10 fg lamda DNA

TroubleShooting Guide
QI want to clone for genomic sequencing.
A Intron's i-StarMAX™ II DNA Polymerase is a DNA Polymerase made with intron's know-how using recombinant DNA technology. It has a nonspecific reaction and error rate And it is very useful to PCR a long template of 20kb or longer.
QI am currently trying to clone TA by obtaining a product from genomic DNA. In my experience, I know that even though pfu is part of cloning, I just went ahead and do not get any results. A tailing possible polymerase recommendation. 
ATA cloning is possible even with our i-StarMAXTM II. However, if you focus on TA cloning, i-Taq™ plus is recommended.
QWhat is the difference between i-StarMAXTM II and i-MAXTM II ?
Ai-StarMAXII ™ is capable of long PCR of 20kb or more with hot start function. On the other hand, i-MAXII ™ has a hot start function It can be used when you want to obtain long size product without having. 
QI am using Maxime i-StarMAXTM II. I want to increase the amount of PCR reaction. Is it OK to increase the amount of reaction by collecting the amount of two tubes into one tube?
AIf you keep the ratio of the amount of reaction, it will not affect the result. However, since the reaction solution may cause sample contamination, proceed with the manual method provided. If you want to control the reaction solution, please purchase 2X PCR Master mix type (25174). 
Related Product
관련제품 Product,No,Capacity,Inquire list
Product Cat.No Capacity inquire
i-StarMAX™Ⅱ DNA Polymerase
25173 250 unit Inquire
2X PCR Master mix Solution (i-StarMAXⅡ)
25174 0.5 ㎖ x 2 vials Inquire
RedSafe™ Nucleic Acid Staining Solution 
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