Maxime™ PCR PreMix (i-pfu)
Dried & aliquoted PreMix Kit with high purity pfu DNA Polymerase with low error rate and Proofreading function which is essential for gene cloning requiring high accuracy
Maxime™ PCR PreMix Kit (i-pfu) contains i-pfu DNA Polymerase and all the components necessary for PCR reaction (dNTP Mixture, reaction buffer, etc.) are included in each PCR tube by ALHP system. It is very excellent in reproducibility because it can reduce the experimental error to pipetting between the experimenters. It is a product with excellent stability of activity due to oxidation and moisture prevention of major components in the product by vacuum compression manufacturing method. In addition, this product can perform PCR by adding template DNA, primer set and D.W only, and it can contain gel loading buffer.
The i-pfu DNA Polymerase used in this product is a typical heat-resistant proof-reading enzyme with 3 '→ 5' exonuclease activity. It has high fidelity and has a low error of 1x10-6 (one error rate per 13,000 base pairs) rate. The proof-reading activity of pfu DNA Polymerase is that when a mismatched nucleotide interferes with DNA polymerase during polymerization, it can be removed and corrected with a complementary nucleotide. This activity allows PCR products of more accurate base sequences to be obtained than Taq DNA Polymerase, but allows amplification of longer DNA templates compared to Taq DNA Polymerase. This is because the mismatched nucleotide interferes with the polymerization reaction. The Pfu DNA Polymerase can remove the DNA polymerase from the template during the DNA synthesis process. It is suitable for long-size PCR (up to ~ 30 kb) which is difficult to amplify. It has high fidelity and proof reading function compared with general Taq DNA Polymerase. As a result, DNA fragments longer than Taq DNA Polymerase (up to 30 kb) can be amplified. Because of these properties, Pfu DNA Polymerase is widely used in experiments where accuracy is required, such as gene cloning and expression, although polymerization efficiency is low and it takes more time to polymerize. The Maxime™ PCR PreMix Kit (i-pfu) has high fidelity, low error rate, excellent stability and reproducibility and has all the components needed for PCR reactions using i-pfu DNA polymerase. It is a dry pellet in the tube, so it can be tested very quickly and easily for various samples.
|Maxime™ PCR PreMix Kit(i-pfu, for 20 μl rxn)||96 tubes|
Amplification test results with i-pfu DNA Polymerase
As a result, it was confirmed that the i-pfu DNA polymerase and 1 Kb (lamda DNA template) and 4.5 Kb (5F' plasmid DNA) amplification were used to confirm the sensitivity of Maxime ™ PCR PreMix Kit (i-pfu) pfu DNA Polymerase.
[ 1 Kb ] Lane M, 1 Kb DNA Marker; lane 1, 200 pg DNA; lane 2, 20 pg DNA, lane 3, 2 pg DNA
[ 4.5 Kb ] Lane M, 1 Kb DNA Marker; lane 1, 50 ng DNA; lane 2, 10 ng DNA, lane 3, 2 ng DNA
MaximeTM PCR PreMix Kit (i-pfu) was tested for the sensitivity test. As a result of checking by 4.5 Kb (5F' plasmid DNA) and 9 Kb (SLT plasmid DNA) amplification, both sizes are superior to those of other products.
[ 4.5 Kb ] Lane M, 1 Kb DNA Marker; lane 1, 200 ng DNA; lane 2, 100 ng DNA, lane 3, 50 ng DNA; lane 4, 25 ng DNA; lane 5, 12.5 ng DNA, lane 6, 6.25 ng DNA
[ 9 Kb ] Lane M, 1 Kb DNA Marker; lane 1, 250 ng DNA; lane 2, 50 ng DNA, lane 3, 10 ng DNA; lane 4, 2 ng DNA; lane 5, 400 pg DNA