DNA
MEGAquick-spin™ Plus Total Fragment DNA Purification Kit
| Cat.No |
Capacity |
Inquire |
| 17290 |
200 col. |
 |
PRODUCT INFORMATION
Description
Product available for performing 65 bp ~ 20 Kb size of DNA extraction and clean up from "PCR product" and "Agarose gel"
- • Recovery of DNA is very high
- PCR clean up : 91 ~ 99%
- Gel extraction : 68 ~ 88%
- • Experiment is very simple and quick
- • Available for downstream applications such as sequencing and restriction enzyme digestion
MEGAquick-spin Plus Total Fragment DNA Purification Kit is used for performing separation/purification of Fragment DNA from PCR product, agarose gel DNA and DNA Clean-up. 65 bp to 20Kb size of DNA can be separated/purified. Furthermore, DNA is purified effectively while removing almost all primer dimer in PCR product. Also, high yield of DNA can be obtained by performing lysis for agarose gel with various concentration. Downstream applications such as DNA sequencing, cloning, labeling, restriction enzyme digestion can be conducted by using high yield of purified DNA.
Applications
- 01For extraction of DNA fragments from agarose gel
- 02For Purification of PCR products or reaction mixtures
Kit Contents
| Contents |
|
200 col. |
| BNL Buffer / (Agarose gel lysis buffer) |
|
160ml |
| Washing Buffer |
|
45ml |
| Elution Buffer |
|
20ml |
| Spin Column |
|
200 ea |
| Collection Tube |
|
200 ea |
| Manual |
|
1 ea |
Technical Data
1) PCR Purification (Comparative test with other company)
As a result of
electrophoresis for PCR product (2.7 kb) purification, the recovery rate was
higher than that of other products, and the primer dimer was effectively
removed.
2) Gel Extraction (Comparative test with other company)
As a result of
the electrophoresis for the purification of the PCR product (218 bp, 2.7 kb), it
was confirmed that the recovery rate was higher than that of other products.
3) PCR & Gel Purification (Recovery rate)
PCR
purification and gel extraction were performed for each size (89 bp ~ 20 kb) of
PCR amplified products. The blue color is the product before amplification, and
the orange graph shows the result after purification. The purification result
for each PCR product confirmed 91 to 99% DNA recovery compared to before
purification, and in the case of the gel extraction product, 68 to 88% DNA
recovery was confirmed compared to the product before purification.
4) Purification results by gel concentration
To check the
performance of the extraction kit according to the agarose gel concentration, a
small fragment sample of 89 bp was used. For the gel concentration, 1.5%, 4%, and 5%
were applied, and each recovery rate result was checked. The recovery rate for
each gel concentration was confirmed to be 72% / 61% / 56%. As a result, it was
confirmed that purification of more than 50% was possible even with a 5% high
concentration gel.
TroubleShooting Guide
- QWhat is recovery rate and what is the amounts of DNA binding capacity?
- AIt is 20µg and recovery rate for gel extraction is 68% ~ 88% and 90% ~ 99% for PCR clean–up.
- QWhat if DNA fragment size is larger than 5kb?
- ABefore centriguation, please warm Elution Buffer / Plus at 60℃.
- QIs it okay to store Kit at Room temperature?
- AThis kit can be stored at Room temperature for 2 years and no damage on performance.
- QWhat is the maximum size of extracted DNA?
- AIt can be 20Kb DNA.
List
