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MEGAquick-spin™ Plus Total Fragment DNA Purification Kit

Cat.No Capacity Inquire
17290 200 col. Inquire

Product available for performing 65 bp ~ 10 Kb size of DNA extraction and clean up from PCR product and agarose gel

  • • Recovery of DNA is very high
       - PCR clean up : 90 ~ 95%
       - Gel extraction : 70 ~ 85%
  • • Experiment is very simple and quick
  • • Available for downstream applications such as sequencing and restriction enzyme digestion

MEGAquick-spinTM Plus Total Fragment DNA Purification Kit is used for performing separation/purification of Fragment DNA from PCR product, agarose gel DNA and DNA Clean-up. 65 bp to 100Kb size of DNA can be separated/purified. Furthermore, DNA is purified effectively while removing almost all primer dimer in PCR product. Also, high yield of DNA can be obtained by performing lysis for agarose gel with various concentration. Downstream applications such as DNA sequencing, cloning, labeling, restriction enzyme digestion can be conducted by using high yield of purified DNA.

  • 01For extraction of DNA fragments from agarose gel
  • 02For Purification of PCR products or reaction mixtures
Kit Contents
Contents 50 col. 200 col.
BNL Buffer / Plus (Agarose gel lysis buffer) 40ml 160ml
Washing Buffer / Plus 15ml 45ml
Elution Buffer / Plus 5ml 20ml
Spin Column 50 ea 200 ea
Collection Tube 50 ea 200 ea
Manual 1 ea 1 ea


Citation List


1 Journal of Genetic Engineering and Biotechnology
Volume 16, Issue 2, December 2018, Pages 305-310
Enhancement of nematicidal potential through cloning and expression of chitinase gene from Bacillus subtilis subsp. Subtilis BTN7A strain Mohamed S. Abdel-Salam a, Hoda H. Ameen b, Abdallah S.M. Kassab c, Ahmed E.A. Mahgoob c, Usama S. Elkelany b Egypt
2 Article: 3452 | Received 11 May 2014, Accepted 30 Aug 2014, Published online: 17 Feb 2016 A comparison between a yeast cell wall extract (Bio-Mos®) and palm kernel expeller as mannan-oligosac-charides sources on the performance and ileal microbial population of broiler chickens Bahman Navidshad, Juan Boo Liang, Mohammad Faseleh Jahromi, Amir Akhlaghi &  Iran
3 Springer, Published: 10 August 2022 Constitutive and extracellular expression of pectin methylesterase from Pectobacterium chrysanthemi in Pichia pastoris Melek Acar & Yagmur Unver  Turkey
4 Clin Exp Vaccine Res. 2022 Jan; 11(1): 12–29. Expression of full and fragment-B of diphtheria toxin genes in Escherichia coli for generating of recombinant diphtheria vaccines Shaimaa Abulmagd,corresponding author1 Abd El-Nasser A. Khattab,2 and Hamdallah Zedan3 Egypt
5 MDPI, Journals  Cosmetics  Volume 9  Issue 1  10.3390/cosmetics9010014 Phytochemical Analysis and Antioxidant, Antimicrobial, and Antiaging Activities of Ethanolic Seed Extracts of Four Mucuna Species Tinnakorn Theansungnoen 1,2,Nichcha Nitthikan 3ORCID,Mayuramas Wilai 1,4,Phanuphong Chaiwut 1,2ORCID,Kanokwan Kiattisin 3,5,* andAekkhaluck Intharuksa 3,* Thailand
6 New Zealand Journal of Marine and Freshwater Research, Received 15 Feb 2022, Accepted 16 Apr 2022, Published online: 04 May 2022 First observations and early life-history aspects of lake rearing galaxiid larvae in the lower Waikato River Basin, New Zealand Bruno O. Davida, Matt Jarvis,Deniz Özkundakci,Josh Smith,Ian C. Duggan,Sung Soo Koh,Jason Augspurger &Tania M. King New Zealand
7 DergiPark Cloning the homologue of gene encoding full-length CBF1 transcription factor from Olea europaea L. leaves Munise YURTSEVER  Maide ŞEKER Şenay VURAL KORKUT  Turkey
8 iScience Discovery of an unrecognized nidovirus associated with granulomatous hepatitis in rainbow trout Sharon Karniely 1 8 10, Adi Faigenboim 2 8, Salsabeel Watted 1, Katia Lapin 1, Eduard Berenshtein 3, Avshalom Hurvitz 4, Arieli Bouznach 1, Ezra Rozenblut 1 9, Massimo Orioles 5, Marco Galeotti 5, Irene Salinas 6, Asaf Berkowitz 1, Eran Bacharach 7, Avi Eldar Israel
9 scientific reports Antimicrobial activity of cell free supernatants from probiotics inhibits against pathogenic bacteria isolated from fresh boar semen Krittika Keeratikunakorn, Thotsapol Kaewchomphunuch, Kampon Kaeoket & Natharin Ngamwongsatit Thailand
10 Springer Genetic differentiation of Megalocytivirus by real time PCR and sequencing Antônio Augusto Fonseca Jr., Mateus Laguardia-Nascimento, Amanda Petrina Scotá Ferreira, Carla do Amaral Pinto, Valdênia Lopes da Silva Gonçalves, Aline Aparecida Silva Barbosa, Anselmo Vasconcelos Rivetti Junior & Marcelo Fernandes Camargos  Brazil
11 Springer An efficient Agrobacterium-mediated system based on the pyrG auxotrophic marker for recombinant expression in the filamentous fungus Penicillium rubens Van-Tuan Tran, Hanh-Dung Thai, Tao Xuan Vu, Ha Hong Vu, Giang Thu Nguyen, Minh Thi Trinh, Huyen Thi Thanh Tran, Huong Thi Thu Pham & Nhung Thi Hong Le  Vietnam




TroubleShooting Guide
QWhat is recovery rate and what is the amounts of DNA binding capacity?
AIt is 20µg and recovery rate for gel extraction is 70% ~ 85% and 90% ~ 95% for PCR clean–up.
QWhat if DNA fragment size is larger than 5kb?
ABefore centriguation, please warm Elution Buffer / Plus at 60℃.
QIs it okay to store Kit at Room temperature?
AThis kit can be stored at Room temperature for 2 years and no damage on performance.
QWhat is the maximum size of extracted DNA?
AIt can be 10Kb DNA.
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