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PCR

FastMix/Frenche™ PCR (i-StarTaq)

Cat.No Capacity Inquire
25411 96 tubes Inquire
PRODUCT INFORMATION
Description

I-StarTaq™ DNA Polymerase's FastMix Kit with high specificity and sensitivity due to hot-start function that reduces the stability at room temperature storage and non-specific amplification 

  • • Maximize stability at room temperature
  • • Stable without change of product activity for more than 6 months at room temperature
  • • Maximize sensitivity and amplification
  • • Increased amplification and sensitivity than existing Maxime products 
  • • High-specificity and accuracy with hot-start
  • • FastMix, All-in-one products
  • • All reagents required for reaction are in dried pellet in one tube 
  • • Immediate PCR by adding template and primer 
  • • Gel loading dye is included for electrophoresis 
  • • Excellent product stability and reproducibility 
  • • Reproducibility with dried% aliquoted FastMix by ALHP system
  • • Excellent stability due to oxidation and moisture prevention by vacuum compression manufacturing method 
  • • Ideal for molecular diagnostic studies 
  • • Applicable to various DNAs from cloned DNA to human genomic DNA

FastMix / Frenche™ PCR (i-StarTaq™) excludes artificial additives for each component of the PCR to optimize PCR activity, maintains activity stability for more than 6 months at room temperature, and provides sensitivity and amplification This is a maximized product. Based on the stability that excludes the influence of the environment, it maintains the constant amplification result and is optimized for diagnostic research.  In addition, this product contains i-StarTaq™ DNA Polymerase to prevent cross-contamination. In addition, all the components (dNTP mixture, reaction buffer, etc.) required for PCR reaction are contained in each PCR tube in the dried pellet state through the ALHP system. By the vacuum compression manufacturing method, oxidation and moisture inhibition This product is very stable in activity.

As mentioned above, since all components are included, when using this product, PCR can be performed by adding template DNA, primer set and D.W only,Gel loading buffer contains Orange G dye that allows gel loading without any other treatment.The i-StarTaq™ DNA Polymerase used in this product has a chemical-based hot-start function that is not antibody-based. It is an enzyme used as a solution when the specific band is weakly amplified by non-specific amplification such as non-specific band or primer by reducing specific amplification and increasing specificity and accuracy. With this hot-start function, it is the best enzyme for general PCR and RT-PCR as well as multiplex PCR and disease diagnosis.FastMix / Frenche ™ PCR (i-StarTaq™) not only maximizes stability at room temperature but also has a much higher sensitivity and amplification rate than existing Maxime products. All components required for PCR reaction are in a dried pellet It is a product that can be tested very quickly and easily for various samples.

Applications
  • 01Pathogen detection
  • 02Complex Genomic DNA PCR
  • 03Complex cDNA template : RT-PCR(Second round PCR of RT-PCR)
  • 04Very low-copy targets
  • 05Reaction with multiplex primer pairs
  • 06Hot-start PCR
  • 07Real-time PCR
  • 08High GC rich, repeat region PCR
  • 09T/A vector cloning
  • 10LOH or MSI analysis related PCR, etc.
TroubleShooting Guide
QI heard that Frenche PCR (i-StarTaq) can be stored at room temperature.
AOur Frenche PCR products incorporate RTS technology to keep the enzyme activity stable for six months at room temperature. The product is valid for 2 years. If you want to use it for a long time, we recommend to keep it frozen.
QI was using the existing Maxime™ PCR PreMix (i-StarTaq). I received the Frenche PCR product through my salesperson, What's the difference with existing products?
AIn the case of our French PCR (i-StarTaq), our product is based on our RTS technology. Its enzyme stability at room temperature is high and enzyme activity is stable for 6 months at room temperature. In addition, the buffer system upgrade maximizes sensitivity and reproducibility.
QCDNA was synthesized with QMaxime RT (Oligo dT). How much cDNA should I put in order to carry out PCR?
AThe amount of cDNA to be used as a template for the APCR reaction is 0.5 to 10% of the initial RT reaction volume. Please refer to the 'PROTOCOL' section of the manual for more information.
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