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PCR

dNTP Mixture (2.5mM)

Cat.No Capacity Inquire
32292 2 ㎖ Inquire
PRODUCT INFORMATION
Description

Nucleotides (> 99%) are available in 2.5 mM

  • • ready-to-use dNTP Mixture
  • • High Purity Purification of Ultra Pure Grade
  • • Small size fragment (<100bp) to long size fragment (<20Kb) amplification   
  • • Provides optimized and stable results of PCR / qPCR   Easy-to-use product

dNTP Mixture (2.5mM) offers Ultra Pure Grade dATP, dCTP, dGTP and dTTP solution mixture dNTP mixture. This product is capable of PCR amplification from low size (less than 100bp) to long size (more than 20kb) and provides optimal results in various molecular biology experiments such as multiplex PCR, real-time PCR and sequencing.

1) dATP Solution (2'-Deoxyadenosine-5'-Triphosphate)
10mM, pH 7.0 in PBS
Ultra Pure Grade
pH 7.0(+/-) 0.5
Stability (-20℃) > 1 year
Max Wavelength 259nm

2) dGTP Solution (2'-Deoxyguanosine-5'-Triphosphate)
10mM, pH 7.0 in PBS
Ultra Pure Grade
pH 7.0(+/-) 0.5
Stability (-20℃) > 1 year
Max Wavelength 253nm

3) dATP Solution (2'-Deoxyadenosine-5'-Triphosphate)
10mM, pH 7.0 in PBS
Ultra Pure Grade
pH 7.0(+/-) 0.5
Stability (-20℃) > 1 year
Max Wavelength 271nm

4) dATP Solution (2'-Deoxyadenosine-5'-Triphosphate)
10mM, pH 7.0 in PBS
Ultra Pure Grade
pH 7.0(+/-) 0.5
Stability (-20℃) > 1 year
Max Wavelength 267nm

Applications
  • 01PCR/qPCR
  • 02Reverse transcription
  • 03DNA labeling
  • 04DNA sequencing, etc
Kit Contents
Content 32292
dNTP Mixture(2.5 mM) 1 ml x 2 tube
Manual 1 ea
Technical Data

conventional PCR comparison test

Our dNTP mix (10 mM) and third-party dNTP Mixture (supplier A, B) were used for long size fragment (20Kb) and Multiplex size fragmentLong size, after amplification, Our Dntp Mixture obtained better results.

Real-time PCR comparison test

Real-time PCR delta Rn, Ct lever showed excellent efficacy as a result of real-time PCR comparison test using our dNTP Set (10 mM) and third-party dNTP Mixture (supplier A).

TroubleShooting Guide
QI have used dNTP in the i-Taq polymerase kit. Can I buy it separately?
AIt is sold as 2.5 mM dNTP mixture and 10 mM dNTP set.For dNTP mixture, 2ml is 125,000 won and 10mM dNTP Set is 1ml for 150,000 won. I used dNTPs during RT-PCR.
QCan I use the 2.5mM dNTP mixture that is sold to Intron?
AYes. Since it is for PCR use, both general PCR and RT-PCR can be used.
QIf you buy a polymerase kit, dNTP is also used. If you are using a third-party DNA polymerase, will dNTP be compatible with other companies?
AdNTP itself does not change its activity depending on the polymerase. Therefore, even if you use a third party polymerase, you can use its dNTP. 
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dNTP Mixture (2.5mM)
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