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RT-PCR

Maxime™ RT-PCR PreMix 

Cat.No Capacity Inquire
25131 96 tubes Inquire
PRODUCT INFORMATION
Description

Dried & aliquoted RT-PCR PreMix Kit for ONE-STEP RT-PCR accurate and fast with high sensitivity, specificity and amplification rate

  • • Convenient One-step RT-PCR
       - Only need to add RNA, gene specific primer and DW of an experimenter
  •  • Sensitive cDNA synthesis and high specificity PCR amplification possible
  •  • Dried & aliquoted PreMix, All-in-one product
       - All components for RT-PCR reaction are in dried pellet in one tube
       - Direct use for PCR by adding template and primer
       - Gel loading dye is included for electrophoresis
  • • High stability and reproducibility
       - Excellent reproducibility with dried & aliquoted PreMix by ALHP (Automatic Liquid Handling Platform) system
       - Protection from oxidation and humidity by vacuum packaging
  • • High specificity and accuracy by hot-start function of Taq DNA polymerase

Maxime™ RT-PCR PreMix Kit is a one-step RT-PCR product that synthesizes cDNA from RNA and amplifies it by PCR. It is a one-step RT-PCR product from cDNA synthesis to PCR amplification in one tube. The one-step RT-PCR method has the advantage of obtaining a higher specificity than 2 steps method, so it is a very useful method for the detection of trace amounts of infectious RNA in a sample. MaximeTM RT-PCR PreMix Kit includes all components (dNTP mixture, Reaction buffer, etc.) necessary for RT-PCR reaction including high-performance OptiScriptTM RTase and hot-start function i- StarTaqTM DNA Polymerase. Since it is supplied in dried pellet state in PCR tubes, it is possible to remarkably reduce experimental errors caused by pipetting among the experimenters. Therefore, the reproducibility is very excellent due to the oxidation and moisture prevention of the main components in the product. Therefore, RT-PCR can be performed at once by adding template RNA, specific primer set and RNase free water only. Gel loading buffer is included for gel loading without any other treatment.

Applications
  • 01RNA qualification study
  • 02RNA expression study
  • 03Specific RNA detection
  • 04Virus research
  • 05Molecular diagnostic study
Kit Contents
Contents 25131
Maxime™ RT-PCR PreMix Kit 96 tubes
Manual 1 ea

[ 참고. Maxime™ RT-PCR PreMix Kit ]

Component in 20 ml reaction

  • OptiScript™ RT System
  • i-StarTaq™ DNA Polymerase
  • RT-PCR Buffer(10x)
  • dNTP Mixture
  • Stabilizer
Technical Data

Observation of RT-PCR efficiency from high concentration RNA to low concentration RNA


 

After cDNA synthesis from 1mg RNA to 1ng RNA, PCR was performed using a housekeeping gene, GAPDH primer. In result, it was confirmed that our Maxime™ RT-PCR Premix Kit is superior to other products.

Lane M, Sizer™100 DNA marker solution; lane 1, 1 mg total RNA; lane 2, 100 ng total RNA; lane 3, 10 ng total RNA; lane 4, 1 ng total RNA

A product optimized for diagnostic research


 

Maxime™ RT-PCR Premix Kit was used to test various RNA viruses such as respiratory viruses for various diagnostic studies. From the results, it was confirmed that the product is optimized for diagnostic research due to its high sensitivity detection performance.

High quality stability and reproducibility


 

MaximeTM RT-PCR PreMix Kit were randomly selected from the latest batch to the batch produced one year ago, and subjected to an active comparative test. As a result, equivalent sensitivity and amplification performance were observed.

Panel A, IBRV virus detection; Panel B, hGAPDH detection

TroubleShooting Guide
QAfter RT-PCR, we will do cloning. 20㎕ seems insufficient in capacity, but I do not think there is a product with 50㎕ capacity. Is it possible to make an order?
AThe Maxime™ RT-PCR PreMix Kit is premix type, and produced in 20㎕ capacity. However, if customer requests 50㎕ capacity, we can customize and supply it.
QI am doing one-step RT-PCR, and I wonder the condition and the concentration of the primer in PCR.
AThe concentration of the primer is usually 10pmol, forward and reverse primer each.
QI would like to try to use the Maxime™ RT-PCR PreMix Kit. Can I use oligo dT instead of gene specific primer?
AThis product is one-step RT-PCR product. Therefore, Maxime™ RT-PCR is not suitable product to use oligo dT primer for RT reaction.
QIn the Maxime™ RT-PCR PreMix Kit, there is optiscript™ RTase in the contents. Is this an AMV or M-MLV?
AThe optiscript™ RTase is a new reverse transcriptase, not AMV and M-MLV.
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