G-spin™ Genomic DNA Extraction Mini Kit (for Bacteria) is designed to efficiently extract genomic DNA from gram-positive or gram-negative bacteria. Normally, manual methods such as alcohol precipitation are used to extract genomic DNA from bacteria, but at this time, you will feel inconvenience about process complexity and low purity. In the case of the homebrew protocol, there are so many different protocols depending on the type of bacteria, which makes it difficult to choose which method to use.

Our G-spin™ Genomic DNA Extraction Mini Kit (for Bacteria) is a dedicated product for genomic DNA extraction of various Bacteria and has been verified by many customers for a long time. An effective and milky Buffer system can stabilize the genomic DNA of bacteria and extract large size genomic DNA without shearing DNA. In addition, it is a product of Spin column type with high purity and high yield GxN technology. It provides the environment that can extract DNA most suitable for research such as PCR, Southern blot, RAPD, Sequencing. ​

2) Genomic DNA isolation from various samples

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G-spin™ Genomic DNA Extraction Kit for Bacteria is ideal for purifying DNA from small amounts of starting materials. For the purpose to obtain optimum DNA yield and quality, do not to overload the sample. It is important. The volume of 1-2 ml is suitable for gDNA prep.(Ideal OD600 value is 0.8-1.1)

Fig. 1. Analysis of gDNA isolated from various samples with G-spin™ Genomic DNA Extraction Kit for Bacteria.
2 μl out of 200 μl of eluent was loaded.

3) Restriction enzyme digestion for isolated genomic DNA ​

Ensure that the carrier DNA dose not interfere with your downstream application. Because of isolated genomic DNA was purified without contamination of protein, RNA, and so on.

Fig. 2. Genomic DNA isolated with G-spin™ Genomic DNA prep Kit is suitable for restriction enzyme digestions.

4) PCR amplification of genomic DNA isolated from bacteria ​

Fig. 3. Agarose gel electrophoresis of PCR products from isolated genomic DNA.

5) Comparison of genomic DNA yield from different company kit ​

In order to compare of genomic DNA yield from competitive company kit, make on experiment. Yield of extraction, G-spinTM Kit stand in different levels compare of competitive company kit.

Fig. 4. Agarose gel electrophoresis of isolated genomic DNA from different company kit.

Applications

• 01Bacteria genome research
• 02PCR, Real time PCR
• 03DNA hybridization : Southern blotting, microarray
• 04Pathogenic bacteria detection
• 05Environmental research

Kit Contents

Contents	50 COLUMNS
Pre-Buffer	3ml
G-Buffer	20ml
Binding Buffer	15ml
Washing Buffer A	9ml
Washing Buffer B(Concentration)	10ml
Elution Buffer	20ml
Spin Column (Green color) & Collection tube	50 columns
RNase A, lyophilized	3 mg
Proteinase K, Lyophilized	1.76mg
Lysozyme, Lyophilized	20mg
Manual	1 ea

 

Citation List

1	Asian-Australas J Anim Sci. 2017 Aug; 30(8): 1099–1104. Published online 2017 Jan 26. doi:  10.5713/ajas.16.0708	Effect of alcohol dehydrogenase 1C (ADH1C) genotype on vitamin A restriction and marbling in Korean native steers	Dong Qiao Peng,1,2 U Suk Jung,1 Jae Sung Lee,1,2 Won Seob Kim,1,2 Yong Ho Jo,1,2 Min Jeong Kim,1 Young Kun Oh,3 Youl Chang Baek,3 Seong Gu Hwang,4 and Hong Gu Lee1,2,*	Korea
2	Applied Microbiology and Biotechnology July 2014, Volume 98, Issue 13, pp 6105–6113	Comparison of droplet digital PCR and quantitative real-time PCR for examining population dynamics of bacteria in soil	Tae Gwan KimSo-Yeon JeongKyung-Suk Cho	Korea
3	J. Microbiol. Biotechnol. (2014), 24(6), 854–861 http://dx.doi.org/10.4014/jmb.1312.12063	Induction of Immune Responses by Two Recombinant Proteins of,Brucella abortus, Outer Membrane Proteins 2b Porin and Cu/ZnSuperoxide Dismutase, in Mouse Model	Kyung Yong Sung1, Myunghwan Jung1, Min-Kyoung Shin1, Hyun-Eui Park1, Jin Ju Lee2 , Suk Kim2, andHan Sang Yoo1,3*	Korea
4	Mustafa, K. “Isolation and Identification of Burkholderia Mallei and Its Susceptibility to Anethum Graveolens Extracts”. ZANCO Journal of Pure and Applied Sciences, Vol. 30, no. 4, Sept. 2018, pp. 11-22, doi:10.21271/ZJPAS.30.4.2.	Isolation and Identification of Burkholderia mallei and its Susceptibility to Anethum graveolens Extracts	Khadija Khalil Mustafa	Iraq
5	16 December 2017|   https://doi.org/10.1002/biot.201700562, Cited by: 9	Biosynthesis of the Nylon 12 Monomer, ω‐Aminododecanoic Acid with Novel CYP153A, AlkJ, and ω‐TA Enzymes	Md. Murshidul Ahsan , Hyunwoo Jeon , Saravanan P. Nadarajan , Taeowan Chung , Hee‐Wang Yoo , Byung‐Gee Kim , Mahesh D. Patil , Hyungdon Yu	Korea
6	01 December 2015, International Journal of Systematic and Evolutionary Microbiology 65: 4674-4681, doi: 10.1099/ijsem.0.000631	Weissella jogaejeotgali sp. nov., isolated from jogae jeotgal, a traditional Korean fermented seafood	Se-Hui Lee1​ ,5,  Hye-Jin Ku1​ ,5,  Min-Ju Ahn1​,  Ji-Sang Hong1​,  Se Hee Lee2​,  Hakdong Shin3​,  Keun Chul Lee4​,  Jung-Sook Lee4​,  Sangryeol Ryu3​,  Che Ok Jeon2​, Ju-Hoon Lee1​	Korea
7	Iraqi Journal of Science, Vol 63 No 6 (2022)	Detection of Efflux Pumps Gene and Relation with Antibiotics Resistance in Uropathogenic Escherichia Coli (UPEC) Isolated from Patients with Cystitis	Hussein O.M. Al-Dahmoshi	Iraq
8	Scientifc reports	Antimicrobial activity of cell free supernatants from probiotics inhibits against pathogenic bacteria isolated from fresh boar semen	Krittika Keeratikunakorn, Thotsapol Kaewchomphunuch, Kampon Kaeoket & Natharin Ngamwongsatit	Thailand
9	Journal of the Hellenic Veterinary Medical Society	Molecular identification and sequencing of Pseudomonas aeruginosa 16S rRNA gene isolated from a variety of raw cow milk samplesin Iraq	AA Saeed, KQ Mayea, SH Shalal	Iraq
10	Springer	Modified gold nanoparticle colorimetric probe-based biosensor for direct and rapid detection of Mycobacterium tuberculosis in sputum specimens	Sara Kooti, Sepide Kadivarian, Ramin Abiri, Parviz Mohajeri, Sara Atashi, Hossein Ahmadpor & Amirhooshang Alvandi	Iran
11	ELSEVIER	Molecular detection of Babesia and Theileria species/genotypes in sheep and ixodid ticks in Erzurum, Northeastern Turkey: First report of Babesia canis in sheep	Ridvan Kirman, Esin Guven	Turkey