G-spin™ Genomic DNA Extraction Mini Kit (for Bacteria) is designed to efficiently extract genomic DNA from gram-positive or gram-negative bacteria. Normally, manual methods such as alcohol precipitation are used to extract genomic DNA from bacteria, but at this time, you will feel inconvenience about process complexity and low purity. In the case of the homebrew protocol, there are so many different protocols depending on the type of bacteria, which makes it difficult to choose which method to use.

Our G-spin™ Genomic DNA Extraction Mini Kit (for Bacteria) is a dedicated product for genomic DNA extraction of various Bacteria and has been verified by many customers for a long time. An effective and milky Buffer system can stabilize the genomic DNA of bacteria and extract large size genomic DNA without shearing DNA. In addition, it is a product of Spin column type with high purity and high yield GxN technology. It provides the environment that can extract DNA most suitable for research such as PCR, Southern blot, RAPD, Sequencing. ​

2) Genomic DNA isolation from various samples

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G-spin™ Genomic DNA Extraction Kit for Bacteria is ideal for purifying DNA from small amounts of starting materials. For the purpose to obtain optimum DNA yield and quality, do not to overload the sample. It is important. The volume of 1-2 ml is suitable for gDNA prep.(Ideal OD600 value is 0.8-1.1)

Fig. 1. Analysis of gDNA isolated from various samples with G-spin™ Genomic DNA Extraction Kit for Bacteria. 2 μl out of 200 μl of eluent was loaded.

 

3) Restriction enzyme digestion for isolated genomic DNA​

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Ensure that the carrier DNA dose not interfere with your downstream application. Because of isolated genomic DNA was purified without contamination of protein, RNA, and so on.

 

 

 

 

Fig. 2. Genomic DNA isolated with G-spin™​ Genomic DNA prep Kit is suitable for restriction enzyme digestions.​

Lane M, 1Kb Ladder DNA Marker, lane 1, E. coli, lane 2, Salmonella pullorum, lane 3, Pseudomonas, lane 4, Corynebacterium spp, lane 5, Staphylococcus aureus, lane 6, Bacillus subtilis.