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DNA

G-spin™ Genomic DNA Extraction Kit (for Bacteria)

Cat.No Capacity Inquire
17121 50 col. Inquire
PRODUCT INFORMATION
Description

Spin type products dedicated to genomic DNA extraction of almost all Gram positive and negative bacteria

    • Quick extraction time
    • Provides all enzymes (RNase A, Lysozyme, Proteinase K) for extraction and is stable in lyophilized
    • Spin column type product without ethanol precipitation process
    • Genomic DNA extraction with high yield and purity is possible
    • Provides all enzymes (RNase A, Lysozyme, Proteinase K) for extraction and is stable in lyophilized ​

G-spin ™ Genomic DNA Extraction Mini Kit (for Bacteria) is designed to efficiently extract genomic DNA from gram-positive or gram-negative bacteria. Normally, manual methods such as alcohol precipitation are used to extract genomic DNA from bacteria, but at this time, you will feel inconvenience about process complexity and low purity. In the case of the homebrew protocol, there are so many different protocols depending on the type of bacteria, which makes it difficult to choose which method to use.

Our G-spin ™ Genomic DNA Extraction Mini Kit (for Bacteria) is a dedicated product for genomic DNA extraction of various Bacteria and has been verified by many customers for a long time. An effective and milky Buffer system can stabilize the genomic DNA of bacteria and extract large size genomic DNA without shearing DNA. In addition, it is a product of Spin column type with high purity and high yield GxN technology. It provides the environment that can extract DNA most suitable for research such as PCR, Southern blot, RAPD, Sequencing.

Applications
  • 01Bacteria genome research
  • 02PCR, Real time PCR
  • 03DNA hybridization : Southern blotting, microarray
  • 04Pathogenic bacteria detection
  • 05Environmental research
Kit Contents
Contents 50 COLUMNS
Pre-Buffer 3ml
G-Buffer 20ml
Binding Buffer 15ml
Washing Buffer A 9ml
Washing Buffer B(Concentration) 10ml
Elution Buffer 20ml
Spin Column (Green color) & Collection tube 50 columns
RNase A, lyophilized 3 mg
Proteinase K, Lyophilized 1.76mg
Lysozyme, Lyophilized 20mg
Manual

1 ea

 

Citation List
1 Asian-Australas J Anim Sci. 2017 Aug; 30(8): 1099–1104.
Published online 2017 Jan 26. doi:  10.5713/ajas.16.0708
Effect of alcohol dehydrogenase 1C (ADH1C) genotype on vitamin A restriction and marbling in Korean native steers Dong Qiao Peng,1,2 U Suk Jung,1 Jae Sung Lee,1,2 Won Seob Kim,1,2 Yong Ho Jo,1,2 Min Jeong Kim,1 Young Kun Oh,3 Youl Chang Baek,3 Seong Gu Hwang,4 and Hong Gu Lee1,2,* Korea
2 Applied Microbiology and Biotechnology
July 2014, Volume 98, Issue 13, pp 6105–6113
Comparison of droplet digital PCR and quantitative real-time PCR for examining population dynamics of bacteria in soil Tae Gwan KimSo-Yeon JeongKyung-Suk Cho Korea
3 J. Microbiol. Biotechnol. (2014), 24(6), 854–861
http://dx.doi.org/10.4014/jmb.1312.12063
Induction of Immune Responses by Two Recombinant Proteins of,Brucella abortus, Outer Membrane Proteins 2b Porin and Cu/ZnSuperoxide Dismutase, in Mouse Model
Kyung Yong Sung1, Myunghwan Jung1, Min-Kyoung Shin1, Hyun-Eui Park1, Jin Ju Lee2
, Suk Kim2, andHan Sang Yoo1,3*
Korea
4 Mustafa, K. “Isolation and Identification of Burkholderia Mallei and Its Susceptibility to Anethum Graveolens Extracts”. ZANCO Journal of Pure and Applied Sciences, Vol. 30, no. 4, Sept. 2018, pp. 11-22, doi:10.21271/ZJPAS.30.4.2. Isolation and Identification of Burkholderia mallei and its Susceptibility to Anethum graveolens Extracts  Khadija Khalil Mustafa  Iraq
5 16 December 2017|
 
https://doi.org/10.1002/biot.201700562, Cited by: 9
 
Biosynthesis of the Nylon 12 Monomer, ωAminododecanoic Acid with Novel CYP153A, AlkJ, and ωTA Enzymes Md. Murshidul Ahsan , Hyunwoo Jeon , Saravanan P. Nadarajan , Taeowan Chung , HeeWang Yoo , ByungGee Kim , Mahesh D. Patil , Hyungdon Yu Korea
6 01 December 2015, International Journal of Systematic and Evolutionary Microbiology 65: 4674-4681, doi: 10.1099/ijsem.0.000631 Weissella jogaejeotgali sp. nov., isolated from jogae jeotgal, a traditional Korean fermented seafood  Se-Hui Lee1​ ,5,  Hye-Jin Ku1​ ,5,  Min-Ju Ahn1​,  Ji-Sang Hong1​,  Se Hee Lee2​,  Hakdong Shin3​,  Keun Chul Lee4​,  Jung-Sook Lee4​,  Sangryeol Ryu3​,  Che Ok Jeon2​, Ju-Hoon Lee1​ Korea
7 Iraqi Journal of Science, Vol 63 No 6 (2022) Detection of Efflux Pumps Gene and Relation with Antibiotics Resistance in Uropathogenic Escherichia Coli (UPEC) Isolated from Patients with Cystitis Hussein O.M. Al-Dahmoshi Iraq
8 Scientifc reports Antimicrobial activity of cell free supernatants from probiotics inhibits against pathogenic bacteria isolated from fresh boar semen Krittika Keeratikunakorn, Thotsapol Kaewchomphunuch, Kampon Kaeoket & Natharin Ngamwongsatit  Thailand
9 Journal of the Hellenic Veterinary Medical Society Molecular identification and sequencing of Pseudomonas aeruginosa 16S rRNA gene isolated from a variety of raw cow milk samplesin Iraq AA Saeed, KQ Mayea, SH Shalal Iraq
10 Springer Modified gold nanoparticle colorimetric probe-based biosensor for direct and rapid detection of Mycobacterium tuberculosis in sputum specimens Sara Kooti, Sepide Kadivarian, Ramin Abiri, Parviz Mohajeri, Sara Atashi, Hossein Ahmadpor & Amirhooshang Alvandi Iran
11 ELSEVIER Molecular detection of Babesia and Theileria species/genotypes in sheep and ixodid ticks in Erzurum, Northeastern Turkey: First report of Babesia canis in sheep Ridvan Kirman, Esin Guven Turkey

 

 

 

TroubleShooting Guide
Q I am trying to extract the genomic DNA of Bacteria, but I am not sure whether it is gram positive or negative. Are there any products available for the iNtRON ?

A We recommend the G-Spin ™ Genomic DNA Extraction Kit (for Bacteria) and refer to the Gram positive bacteria protocol.
 
Q What is the role of Pre-buffer in order to use only Pre-buffer for Gram-positive bacteria protocol?

A Gram positive bacteria have thicker peptidoglycan layers than negative bacteria. Through an enzyme such as Lysozyme,
It is necessary to disassemble, and Pre-buffer can decompose the thick outer wall of Gram positive.

Q Is there a way to increase the yield of genomic DNA?

A When using this product, increase the lysis time to complete the lysis process, and reduce the elution volume to obtain a higher recovery rate.                                    

Q When I put EtOH in washing buffer, I did not have absolute EtOH, so I put 80% EtOH. Is it okay?

A The use of Absolute EtOH ensures complete removal of the salt, maximizing the efficiency of the washing step.​
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