Description
TaqMan Probe type, one-step RT-PCR product for quantitative PCR
The RealMOD™ Probe W2 2x qRT-PCR mix is a one-step qRT-PCR product using RNA. The RT-PCR Enzymes included in the product are RTase which is responsible for the reverse transcription reaction to synthesize cDNA from RNA and has MMLV RH (-) RTase. DNA polymerase, which is dedicated to PCR reaction to amplify a large amount of synthesized cDNA, has a hot-start function and is mixed with a composition showing optimal activity. This product is provided in 2x type, and reaction buffer, dNTP, and Mg2+ + required for one-step qRT-PCR reaction are combined in the optimum ratio, so users can analyze reproducible results. It is compatible with various real-time PCR equipment and can be used according to the capacity of each equipment.
• Products with excellent activity due to lack of RNaseH activity that degrades RNA / DNA hybrid
• High specificity of hot-start DNA polymerase is applied to one-step RT-PCR
• High reproducibility and convenience in qRT-PCR experiments
The RealMOD™ Probe W2 2x qRT-PCR mix is a one-step qRT-PCR product using RNA. The RT-PCR Enzymes included in the product are RTase which is responsible for the reverse transcription reaction to synthesize cDNA from RNA and has MMLV RH (-) RTase. DNA polymerase, which is dedicated to PCR reaction to amplify a large amount of synthesized cDNA, has a hot-start function and is mixed with a composition showing optimal activity. This product is provided in 2x type, and reaction buffer, dNTP, and Mg2+ + required for one-step qRT-PCR reaction are combined in the optimum ratio, so users can analyze reproducible results. It is compatible with various real-time PCR equipment and can be used according to the capacity of each equipment.
Applications
•01Real-time PCR : Detection and quantification of DNA and cDNA targets
•04Gene knockdown verification
•03Array validation
•04Gene knockdown verification
Kit Contents
| Content |
Volumes |
| RealMOD™ Probe W² 2x qRT-PCR mix |
100 rxn. |
| Manual |
1 ea |
Wide Instruments Compatibility
RealMOD ™ Probe W2 2x qRT-PCR mix is standard qPCR enabled. The types of devices that can be tested using this product are as follows.
- • Applied BioSystems : QuantStudio™ 12K Flex, ViiA™ 7, 7900HT, 7500, 7700, StepOne™ & StepOnePlus™
- • Stratagene : MX3000P™, MX3005P™
- • Bio-Rad : CFX96™ & CFX384™, iQ™5 & MyiQ™, Chromo4™, Opticon
E=mc®2 & MiniOpicon®
- • Qiagen : Rotor-Gene® Q, Rotor-Gene® 6000
- • Eppendorf : Mastercycler® ep realplex2 & ep realplex4
- • Illumina : The Eco™
- • Roche : LightCycler®480
Technical Data
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Comparison data of performance by using RealMODTM Probe W2 2x qRT-PCR mix
