iNtRON Biotechnology DR

Real-time PCR

RealMOD™ Probe R² 2X qPCR mix (with UDG)

Cat.No	Capacity	Inquire
25363.200	200 rxn	Inquire
25363.500	500 rxn	Inquire
25363.1000	1000 rxn	Inquire

Inquire List

Description
Trouble Shooting
Guide
Related products
Protocol
MSDS

PRODUCT INFORMATION

Description

A product of 2x Master Mix that can easily perform TaqMan Probe-type Real-time PCR

• Minimize cross contamination by applying UDG

• Multiplex PCR of up to 5 channels possible

• Fast PCR protocol can be applied within 50 minutes

• Real-time PCR reaction volume control with 2X Master Mix type

• Enhanced PCR efficiency of Taqpolymerase through application of Anti-Taqantibody

• Excellent reproducibility and enhanced PCR performance stability against interferences

RealMOD ™ Probe R² 2X qPCR mix (with UDG) is a TaqMan Probe type product that enables quantitative PCR using DNA. The UDG-system is applied, and the product amplified through this product includes Uracil. Therefore, in the second experiment, the DNA containing Uracil can be degraded by UDG-syste to significantly reduce false positives for PCR amplicon. In addition, this product has a hot-start function, so the dimer generation rate is low, and the reproducibility and stability against interfering substances are high. The qPCR results are excellent, and it is provided in 2X MasterMix type, so the capacity can be adjusted according to the conditions of use.

Applications

- Preventing cross/carry over contamination

- Detection and quantification of DNA target

- Gene-expression analysis

- Pathogene detection and quantification

Kit Contents

Content	Volumes
25363.200	200T
25362.500 / 1000	500T / 1000T

Technical Data

Figure 1. Performance comparison between normal and fast protocols using RealMOD™ Probe R² 2x qPCR mix (with UDG).

Applying fast PCR condition using RealMOD™Probe R2 2X qPCR (with UDG), the same detection performance was confirmed compared to normal PCR conditions.

-Template : Treponema varginallis DNA 10-fold dilution serially

-(A) Normal PCR condition (96 min) (B) Fast PCR condition (50 min)

Figure 2. Multiplex PCR of various DNA using RealMOD™ Probe R² 2x qPCR mix (with UDG).

The Multiplex PCR amplification performance of the RealMOD™ Probe R² 2X qPCR mix was evaluated using various types of viral DNA by 10-fold dilution serially (Using CFX-96 Real-time PCR system).

(A: C.trachomatisDNA , B: N.gonorrhoeaeDNA, C: T.vaginalisDNA, D: M.genitaliumDNA, E : 4 plexMultiplex PCR)

Wide Instruments Compatibility

RealMOD™ Probe R² 2X qPCR mix (with UDG) is a standard qRT-PCR product.

The types of devices that can be tested using this product are as follows.

• Applied BioSystems : QuantStudio™ 12K Flex, ViiA™ 7, 7900HT, 7500, 7700, StepOne™ & StepOne plus™

• Stratagene : MX3000P™, MX3005P™

• Bio-Rad : CFX96™ & CFX384™, iQTM5 & MyiQ™, Chomo4™, Opticon® 2 & MiniOpicon®

• Qiagen : Rotor-Gene® Q, Rotor-Gene® 6000

• Eppendorf : Mastercycler®, ep realplex2 & ep realplex4

• Roche : LightCycler® 480

• Illumina : The Eco™

TroubleShooting Guide

Q. As a result of the experiment, amplification cannot be confirmed.

A. First, check that there is no problem with the template extraction process. Second, make sure that there are no missing contents (primer, probe, 2X PCR mix) when preparing the PCR mixture. Third, check the experimental conditions by reviewing the annealing temperature of the primer design and prime.

Q. As a result of the experiment, a signal was detected in the NC (negative control).

A. First, since non-specific reactions may occur due to environmental factors, please cleanly disinfect the equipment and experiment table you are using before proceeding. Second, since non-specific reactions may occur due to experimental factors (contamination by the template to be used), please make a mixture, dispense negative control first, and then proceed with the experiment on the target.

Q. Is ROX dye added to the product?

A. ROX dye is not added to this product, and it must be used after adding it if necessary. If there is a selection for passive reference dye during the experiment, please proceed with none.