Description

Comprehensive one-step qRT-PCR premix for ultra-sensitive RNA quantification with integrated contamination control.

• • Combines reverse transcription and qPCR in a single-tube reaction for streamlined workflow
• • Includes 5X gDNA Eraser for rapid genomic DNA removal within 2 minutes
• • Built-in dUTP/UDG system ensures 99.99% prevention of carryover contamination
• • Pre-mixed Universal ROX enables compatibility with all qPCR instruments
• • Blue tracking dye allows visual confirmation of pipetting accuracy
• • Dual-lock enzyme system ensures high specificity and reproducibility

RealMOD™ Green T² 2X qRT-PCR mix is a comprehensive premix designed for ultra-sensitive one-step RNA quantification. By integrating reverse transcription and qPCR into a single reaction, it simplifies the workflow while maintaining high data reliability. The included 5X gDNA Eraser efficiently removes contaminating genomic DNA within 2 minutes without affecting RNA integrity, eliminating false-positive signals. Additionally, the dUTP/UDG system prevents carryover contamination with 99.99% efficiency, ensuring accurate and reproducible results. Universal ROX and a blue tracking dye provide instrument compatibility and visual pipetting verification.

Applications

• 01One-step detection and quantification of cellular and viral RNA
• 02Gene expression analysis with integrated gDNA removal
• 03High-throughput diagnostic research requiring strict contamination control

Kit Contents

Technical Data

RealMOD™ Green T² 2X qRT-PCR mix delivers unmatched amplification efficiency, exceptional sensitivity, and reliable performance across diverse RNA templates, including both cellular and viral targets.

Performance Comparison against Competitor (GAPDH Target)

•​ Experimental Assay Criteria: Amplification curves and melt peaks of the house-keeping GAPDH gene from K562 cellular total RNA.

•​ Dilution Matrix Parameters: The RNA template was 10-fold serially diluted from 20 ng down to 200 pg (executed in duplicate).

•​ Performance Validation: The RealMOD™ Green T² 2X qRT-PCR mix demonstrates significantly earlier Ct values, higher final fluorescence intensities, and specific single-target amplification profile compared to Supplier A.

Linear Dynamic Range for Viral Copy Targets (CPIV RNA)

•​ ​Verification of the broad linear dynamic range and high-sensitivity quantification matrix utilizing 10- fold serially diluted CPIV viral RNA samples (6 ng down to 6 pg) in duplicate amplification kinetics.

•​ ​Experimental Assay Criteria: Amplification curves of viral CPIV RNA targets.

•​ ​Dilution Matrix Parameters: The viral template architecture was 10-fold serially diluted from 6 ng down to 6 pg (executed in duplicate).

•​ ​Performance Validation: The evenly spaced amplification curves demonstrate excellent amplification efficiency and highly sensitive detection capability even at ultra-low RNA concentration thresholds, validating the kit's broad compatibility and performance stability with diverse, low-copy RNA types.

To evaluate the anti-contamination performance of the active dUTP/UDG system in the RealMOD™ Green T² 2X qRT-PCR mix.

Amplification was performed using templates completely substituted with Uracil (all-U) to simulate aerosol carryover contaminants, alongside normal Thymine (all-T) templates representing true targets.

The test was conducted across a dynamic template input range of 4 pg, 40 pg, and 400 pg.