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Versatile SYBR Green Solution

Date

01-Aug-26

Hit

3109

New Product Series

RealMOD™ Green T2 Series

Perform with Confidence — High-Performance Real-Time PCR Solutions Backed by Proven Data

qPCR Master Mix · DNA Targets qRT-PCR Master Mix · RNA Targets

92%

Target Specificity

Universal ROX

Compatible with any equipment

< 40 min

One-Step Total Runtime

99.99%

Carryover Prevention

Product Lineup at a Glance

RealMOD Green T2 2X qPCR mix Product Shot

DNA Target

RealMOD™ Green T² 2X qPCR mix

Cat. No. 25364 · 200 / 500 rxns

Optimized specifically for genomic DNA and cDNA templates.
Antibody-mediated hot-start Taq polymerase completely suppresses non-specific primer-dimer formation.
Delivers highly linear amplification sensitive down to single-digit target copies.

RealMOD Green T2 2X qRT-PCR mix Product Shot

RNA Target

RealMOD™ Green T² 2X qRT-PCR mix

Cat. No. 25365 · 100 / 300 rxns

Convenient one-tube, one-step workflow integrating reverse transcription and qPCR.
Pre-blended with 5X gDNA Eraser to completely eliminate genomic DNA contamination prior to run.
Integrated dUTP/UDG system effectively blocks PCR carryover contamination.

Advanced Chemistry & Core Formulations

The RealMOD™ Green T² Series incorporates an advanced chemistry matrix designed to eliminate sample dropouts at low template concentrations while suppressing non-specific, template-independent amplification. By integrating specialized enzyme stabilizers with a high-performance buffer system, this series guarantees absolute data integrity for critical research and molecular diagnostics.

Universal Instrument Compatibility

Pre-blended with a universal ROX passive reference dye. Performs seamlessly across low-ROX, high-ROX, and ROX-independent qPCR instruments without manual optimization.

Visual Verification Dye

Formulated with a non-interfering inert blue tracking dye for rapid visual confirmation of pipetting accuracy in multi-well plates, drastically reducing human loading errors.

Streamlined Two-Step Setup

Simply add primers and templates directly to the 2X master mix. Ready for immediate thermocycling, eliminating complex preparation protocols.

Workflow Comparison & Acceleration

1. Sample Prep
DNA/RNA Extraction

2. RT Reaction
3 Minutes (Fast RT)

3. Amplification
Fast qPCR Run

4. Analysis & Results
Total < 40 Minutes

Conventional Workflow: ~80+ Min ➔ RealMOD™ Green T²: < 40 Min (Save over 1 hour of total runtime)

Data-Driven Performance Validation

▶ RealMOD™ Green T² 2X qPCR mix

Performance Comparison vs. Competitor (GAPDH Target)

(A) Amplification Plot (FAM Channel)

(B) Melting Curve Profile

Comparison of 10-fold serial dilutions (5 ng to 50 pg) of human gDNA template: RealMOD™ Green T² demonstrates significantly earlier Ct values, higher plateau fluorescence intensity, and cleaner single-target amplification profiles compared to Supplier A.

Linear Dynamic Range and Target Specificity

Standard Curve (Linearity)

R² = 0.9994 | E = 99.5%

Target Specificity Comparison

92% Specificity

Amplification Plot (CPIV cDNA)

Single-copy detection

▶ RealMOD™ Green T² 2X qRT-PCR mix

Performance Comparison vs. Competitor (K562 Total RNA, GAPDH Target)

(A) Amplification Plot (FAM Channel)

(B) Melting Curve Profile

Comparison of 10-fold serial dilutions (20 ng to 200 pg) of K562 cell total RNA: RealMOD™ Green T² 2X qRT-PCR Mix delivers earlier Ct values, superior final fluorescence yield, and high-specificity amplification curves compared to Supplier A.

Linear Dynamic Range & Carryover Contamination Control

Linear Dynamic Range for Viral Target (CPIV RNA)

Dilution Range: 6 ng to 6 pg

Carryover Contamination Control (dUTP/UDG System)

99.99% Prevention Efficiency

Ordering Information

Category	Cat. No.	Product Name	Capacity
Green Type	25364	RealMOD™ Green T² 2X qPCR mix	200 / 500 rxns
Green Type	25365	RealMOD™ Green T² 2X qRT-PCR mix	100 / 300 rxns
Probe Type	25363	RealMOD™ Probe R² 2X qPCR mix (with UDG)	200 / 500 / 1,000 rxns
	25362	RealMOD™ Probe R² 2X qRT-PCR mix (with UDG)	100 / 500 / 1,000 rxns
	25361	RealMOD™ Probe M² 2X qRT-PCR mix (with UDG)	100 / 500 / 1,000 rxns
	25360	RealMOD™ Probe M² 2X qPCR mix (with UDG)	100 / 500 / 1,000 rxns
Extraction Kits (Associated Products)	17045/17046	G-spin™ Total DNA Extraction Mini Kit	50 / 200 col.
	17221	easy-spin™ Total RNA Extraction Kit	50 col.
	17156	Patho Gene-spin™ HS DNA/RNA Extraction Kit	50 col.

iNtRON Biotechnology, Inc.

Global Leader in Molecular Diagnostics & Gene Extraction

Explore the products in detail >>

Technical Support: tech@intronbio.com
Tel: 82-31-739-5741 | Fax: 82-31-5264

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