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iNRON Magazine

Versatile SYBR Green Solution
Date 01-Aug-26 Hit 3172
iNtRON Biotechnology NEW HIGH-PERFORMANCE MASTER MIX
RealMOD(TM) Green T(2) Series
High-Specificity Real-Time PCR
Antibody-mediated hot-start Taq polymerase completely suppresses non-specific primer-dimers.
Experience superior sensitivity down to single-digit copies with universal instrument compatibility.
qPCR - DNA TARGET qRT-PCR - RNA TARGET
92%
Target Specificity
Universal ROX
All Equipment Ready
< 40 min
Rapid 1-Step Protocol
99.99%
Carryover Prevention
REALMOD(TM) HIGH-PERFORMANCE REAL-TIME PCR
Engineered to Eliminate False Positives and Primer-Dimers
RealMOD(TM) Green T(2) 2X Master Mix provides high-efficiency amplification for DNA templates,
while RealMOD(TM) Green T(2) 2X qRT-PCR integrates reverse transcription and qPCR in a single tube.
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Product Lineup at a Glance
Optimized 2X master mix formulations designed for robust gene quantification from gDNA, cDNA, or total RNA.
RealMOD(TM) Green T(2) 2X qPCR mix Product Shot
DNA Target - SYBR Green I
RealMOD(TM) Green T(2) 2X qPCR mix
Cat. No. 25364 - 200 / 500 rxns
Template
Genomic DNA (gDNA), cDNA libraries, plasmid DNA
Polymerase
Antibody-mediated hot-start Taq polymerase
Sensitivity
Linear amplification down to 6 target copies
Features
-Universal ROX passive reference dye pre-blended
-High PCR efficiency (> 90%) across GC-rich amplicons
-Fast cycling protocol completed within 15-20 min
View Product Details ->
RealMOD(TM) Green T(2) 2X qRT-PCR mix Product Shot
RNA Target - 1-Step One-Tube
RealMOD(TM) Green T(2) 2X qRT-PCR mix
Cat. No. 25365 - 100 / 300 rxns
Template
Total RNA, viral RNA, in vitro transcripts (1 pg - 1 ug)
Enzyme Mix
M-MLV Reverse Transcriptase + Hot-Start Taq Polymerase
Contamination
dUTP/UDG carryover prevention & gDNA Eraser included
Features
-Seamless 1-step cDNA synthesis and real-time qPCR
-Pre-mixed 5X gDNA Eraser eliminates genomic DNA background
-Complete reaction runtime under 40 minutes
View Product Details ->
2
Core Technological Innovations
Solving traditional SYBR Green limitations with antibody-mediated specificity and universal instrument adaptability.
SPECIFICITY
Antibody Hot-Start
Affinity-purified anti-Taq antibodies tightly lock polymerase activity at room temperature, suppressing non-specific annealing and primer-dimer formation.
COMPATIBILITY
Universal ROX
Formulated with an optimized universal reference dye concentration, enabling seamless operation on Low-ROX, High-ROX, and No-ROX real-time instruments without separate adjustments.
INTEGRITY
UDG & gDNA Eraser
Thermally labile Uracil-DNA Glycosylase (UDG) degrades previous dUTP-amplified carryover amplicons, while 5X gDNA Eraser digests background genomic DNA within 2 minutes.
3
Ordering Information
Category Cat. No. Product Name Size
Green qPCR 25364 RealMOD(TM) Green T(2) 2X qPCR mix 200 / 500 rxns
Green qRT-PCR 25365 RealMOD(TM) Green T(2) 2X qRT-PCR mix 100 / 300 rxns
Probe qPCR 25363 RealMOD(TM) Probe R(2) 2X qPCR mix (with UDG) 200 / 500 rxns
Probe qRT-PCR 25362 RealMOD(TM) Probe R(2) 2X qRT-PCR mix (with UDG) 100 / 500 rxns
iNtRON Biotechnology
13202, 137, Sagimakgol-ro, Jungwon-gu, Seongnam-si, Gyeonggi-do, Republic of Korea
Global HQ: iNtRON Biotechnology, Inc. | Tel: +82-31-739-5741 | Fax: +82-31-739-5264
Technical Support: tech@intronbio.com

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